Contact an Account ManagerSolid phase extraction with automated elution and HPLC of torsemide and metabolites from plasma
Summary
Type: HPLC
Application #: 104960
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV, 290nm
Conditions: eluent A: 0.1M KH2PO4 pH 4.5 - acetonitrile (86.5:13.5, v/v); eluent B: 0.1M KH2PO4 pH 4.5 - acetonitrile (70:30, v/v)
gradient: 0 - 15min, 99% A; 15.1 - 18min, 1% A; 18.1 - 30min, 50% A; from 30.1 min, 99% A
flow rate 1.5ml/min
temperature 45°C
Authors: Karnes HT
Source: J Liquid Chromatogr 12 (1989) 1809 - 1818
Details
Legend: Fig. 2. Chromatogram of blank plasma subjected to the torsemide extraction
procedure.
Fig. 3. Chromatogram of spiked blank plasma showing peaks for torsemide (tr =
27.5min, 25ng/ml), M1 (tr = 15.0m